The sequel — Ai Cho Aniki which translates to "Love Super Big Brother" — was developed by Masaya and also released for the PC Engine. In this game, players controlled Samson and Adon directly. A key difference in the control scheme of Ai Cho Aniki is that instead of rapidly pressing buttons as a means of firing at enemies, players now had to input intricate button combinations, Street Fighter-style. By and large, Cho Aniki and Ai Cho Aniki play very similarly.
In the second game, Ai Cho Aniki, Bo Emperor Conshyasu (BoteiConshyasu, a pun of "body conscious") raise the Neo Builder Army to fight Idaten and Benten. However, the two fall in love and elope. This leaves Samson and Adon to fight the new threat, Su Emperor Roido (SuTeiLoido, pun of steroid). This time Taku Iwasaki scored the soundtrack.
The game was released on Nintendo's Virtual Console service in 2007 in Japan.
Source: Wikipedia, "Cho Aniki", available under the CC-BY-SA License.