Katherine Marlowe is the primary antagonist of Uncharted 3: Drake's Deception and is voiced by Rosalind Ayres. Marlowe is the coldly calculating leader of a secret society whose roots date back over four hundred years to the court of Queen Elizabeth I. She has a long-standing rivalry with Nathan Drake over Sir Francis Drake’s ring – something they both contend is rightfully theirs and is also the key to an ancient mystery. Katherine Marlowe is a much more cerebral enemy than Drake has confronted in the past, using both psychological and physical tactics to get what she is after. Marlowe also has the assistance of her team of highly-trained agents in this secret society – they are far more elusive and dangerous than the hired guns Nathan Drake and Victor Sullivan have faced before. She treats her subordinates with slight disdain, getting annoyed with Talbot for hiring Charlie Cutter. Marlowe appears to have studied Nathan's past and reveals that he's not actually the direct decedent of Sir Francis Drake - he was merely in an orphanage named after the great explorer. She first meets a teenage Drake in Colombia, forcing him to give her the Cipher disk he had just stolen from a museum to her but fails to get Sir Francis Drakes ring when he runs off. Twenty years later, she meets with Drake in an Alleyway and steals his ring. However, she soon discovers it's a fake and promptly loses both the disc and the ring when Drake steals both. Marlowe then sends Talbot and her agents to France and Syria, successfully getting the clues to the location of Ubar. She then captures Nate and Sully and leaves for the lost city. Nathan eventually finds her using a winch to drag out King Solomon's hallucinogenic Talisman from the lakes in Ubar and destroys the foundations of the city, causing Marlowe to fall into a pool of quicksand. She goads Drake to save her, asking if he can earn his surname but is promptly consumed by the quicksand.
Source: Wikipedia, "List of Uncharted characters", available under the CC-BY-SA License.